Development and Validation for Simultaneous Estimation of Perindopril and Indapamide by UPLC-UV in Tablet Dosage form

An ultra-performance liquid chromatography (UPLC) method with UV detection was developed for simultaneous estimation of Perindopril and Indapamide in tablet dosage form of pharmaceutical formulation. Simultaneous determination of both drugs is highly significant as this would tolerate more effective generation of clinical data and could be more cost-effective than distinct assays. The chromatography was carried out at 25 °C using an isocratic mobile phase consisting of buffer (0.1% ortho phosphoric acid in Water v/v) and acetonitrile in the ratio of 45:55% (v/v) using variable wavelength UV– Vis detector set at 254nm. Perindopril and Indapamide were eluted isocratically at a steady flow rate of 0.3mL/min, indicated reasonable good assay parameters. The retention times were around 0.8 min and 1.1 min, asymmetry factors 1.7 and 1.6, and linearity correlation coefficients were in the range 20 - 120 and 6.25 - 37μg/ml, respectively. Regression coefficient was
0.999 for both the compounds, while recovery from samples were 98.09-100.17 and 99.06–99.99% for Perindopril and Indapamide, respectively. Relative standard deviations (RSD) of intra- and inter-day precisions were < 2% for both the drugs. Specificity/selectivity experiments revealed the absence of interference from excipients. From the above parameters indicated that the present developed method is precise, accurate, reproducible and specific for desired drug analysis. Moreover, it can also be used for routine simultaneous
estimation of Perindopril and Indapamide in combinations tablets of pharmaceutical drug products.