Extractability and physicochemical properties of cobia ( Rachycentron canadum ) skin collagen as influenced by lactic acid concentration

Herein, we demonstrate why deeming diffusible reactive oxygen species (DROS) as toxic wastes does not afford a comprehensive understanding of cytochrome P450 mediated microsomal xenobiotic metabolism (mXM). Using the recent insights unveiled in mechanistic redox enzymology, we reason out the remaining pieces of the mXM mechanistic chemistry and
support our proposals with visual evidence and thermodynamic calculations. Particularly, we elucidate how murburn model better explains- (i) promiscuity of the unique P450-reductase; (ii)
prolific activity and inhibitions of CYP3A4; (iii) structure-function correlations of important key CYP2 family isozymes- 2C9, 2D6 and 2E1; and (iv) mutation studies and mechanism-based
inactivation of CYPs. In the light of our findings, it is opportune to revamp the methodologies employed for screening potential drug candidate from lead molecules.