Insilico drug repurposing using FDA approved drugs against Membrane protein of SARS-CoV-2

In the present study, biosorption of lead using green algae as biosorbent from aqueous solution was studied with one-factor-
at-a-time (OFAT) method. The batch biosorption process is a function of agitation time, biomass loading, initial concentration
of lead and solution pH. Further, face-centered central composite design under RSM application was applied to optimize the process parameters to get more percentage biosorption of lead. The percentage removal of lead was found to be 71% in batch experiment by OFAT method, whereas in RSM study the maximum biosorption rate 75% was reached at optimum conditions of agitation time 40 min., dosage 3 g, initial ion concentration 15 mg/L and pH 3.35. The adsorbent was char-acterized by FTIR, scanning electron microscopy (SEM) and Brunauer–Emmett Teller (BET) analysis. The kinetic models
were also analyzed to determine the biosorption mechanism. It was found that the data best followed to pseudo second order
mechanism. Biosorption isotherms were successfully ftted by Langmuir isotherm (R2=0.974) and d) Freundlich isotherm
(R2=0.983) models.